Anticorps Polyclonal de lapin anti-FANCA

• Phare
• Validé par KD/KO

FANCA Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 11975-1-AP

Synonymes

FA, FA1, FAA, FACA, FAH

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Galerie de données de validation

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  WB analysis of HeLa cells using 11975-1-AP

  WB results of FANCA (11975-1-AP, 1:500) with si-control and si-FANCA transfected HeLa cells.

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  WB analysis using 11975-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11975-1-AP (FANCA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human stomach cancer using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse intestine using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de l'estomac humain, tissu de cancer du col de l'utérus humain, tissu de thymus de souris, tissu d'intestin grêle de rat, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11975-1-AP cible FANCA dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FANCA Protéine recombinante Ag2598
• Nom complet: Fanconi anemia, complementation group A
• Masse moléculaire calculée: 163 kDa
• Poids moléculaire observé: 163 kDa
• Numéro d’acquisition GenBank: BC008979
• Symbole du gène: FANCA
• Identification du gène (NCBI): 2175
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FANCA, also named as FAA, FACA and FANCH, is a DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

Publications

Species	Application	Title
	WB	Nat Struct Mol Biol; Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.; Authors - Xinlin Xu ; KO Validated
mouse	WB	EMBO Mol Med; WNT inhibition creates a BRCA-like state in Wnt-addicted cancer.; Authors - Amanpreet Kaur
human	WB	Cell Rep; C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair; Authors - Zenan Zhou
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai); FANCA facilitates G1/S cell cycle advancement, proliferation, migration and invasion in gastric cancer; Authors - Wei Wang ; KD Validated
human	WB	Gene; Circular RNA Circ_0000119 promotes gastric cancer progression via circ_0000119/miR-502-5p/MTBP axis; Authors - Mengyan Xie
human	IP,IHC,IF	Gene; Identification of rare heterozygous missense mutations in FANCA in esophageal atresia patients using next-generation sequencing.; Authors - Yu Feng

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