Anticorps Recombinant de lapin anti-FANCD2

FANCD2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241048G8

N° de cat : 83946-4-PBS

Synonymes

241048G8, FA4, FACD, FAD, FAD2

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Galerie de données de validation

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  WB analysis using 83946-4-RR (same clone as 83946-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83946-4-RR (FANCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83946-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83946-4-RR (same clone as 83946-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCD2 antibody (83946-4-RR, Clone: 241048G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin. This data was developed using the same antibody clone with 83946-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83946-4-RR (same clone as 83946-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FANCD2 Recombinant antibody (83946-4-RR, Clone:241048G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83946-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83946-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83946-4-RR against Human FANCD2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83946-4-PBS cible FANCD2 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FANCD2 Protéine recombinante Ag29460
• Nom complet: Fanconi anemia, complementation group D2
• Masse moléculaire calculée: 164 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: NM_001018115
• Symbole du gène: FANCD2
• Identification du gène (NCBI): 2177
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Amongst the FA genes, Fanconi Anemia Group D2 Protein (FANCD2) is the most evolutionarily conserved gene, demonstrating a high sequence similarity from lower eukaryotes to humans. Upon activation, Fanconi anemia group D2 protein (FANCD2) is monoubiquitinated at K561 and works in cooperation with other FA and non-FA proteins to repair DNA damage. In regards to the FA pathway, monoubiquitinated or activated FANCD2 is often portrayed as the functional representative for the functions of activated FA signaling. (PMID: 28825622)

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