Anticorps Polyclonal de lapin anti-FANCG

FANCG Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10215-1-PBS

Synonymes

XRCC9, Protein FACG, Fanconi anemia group G protein, FAG, DNA repair protein XRCC9

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Galerie de données de validation

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  WB analysis of Jurkat using 10215-1-AP (same clone as 10215-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 10215-1-AP (same clone as 10215-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10215-1-AP (same clone as 10215-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 10215-1-AP (same clone as 10215-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10215-1-AP (same clone as 10215-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 10215-1-AP (same clone as 10215-1-PBS)

  IP result of anti-FANCG (IP:10215-1-AP, 4ug; Detection:10215-1-AP 1:300) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10215-1-AP (same clone as 10215-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCG antibody (10215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10215-1-PBS cible FANCG dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FANCG Protéine recombinante Ag0249
• Nom complet: Fanconi anemia, complementation group G
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 65-68 kDa
• Numéro d’acquisition GenBank: BC000032
• Symbole du gène: FANCG
• Identification du gène (NCBI): 2189
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FANCG, also named as XRCC9, is a vDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's a candidate tumor suppressor gene. FANCA and FANCG are critical for the localization of the FANC core complex. FANCA and FANCG form a stable complex with a molecular weight of ∼500 kDa that is predicted to be a heterotetramer (PMID: 32002546).

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