Anticorps Monoclonal anti-FANCL

• Phare
• Validé par KD/KO

FANCL Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

6E2B6

N° de cat : 66639-1-Ig

Synonymes

FAAP43, FANCL, Fanconi anemia group L protein, PHF9, POG

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Galerie de données de validation

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  WB analysis of HeLa using 66639-1-Ig

  HeLa cells and HEK-293 cells were subjected to SDS PAGE followed by western blot with 66639-1-Ig (FANCL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66639-1-Ig

  WB result of FANCL antibody (66639-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FANCL transfected HEK-293 cells.

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  IF Staining of HeLa using 66639-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCL antibody (66639-1-Ig, Clone: 6E2B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below

Informations sur le produit

66639-1-Ig cible FANCL dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FANCL Protéine recombinante Ag6192
• Nom complet: Fanconi anemia, complementation group L
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 41-43 kDa
• Numéro d’acquisition GenBank: BC054517
• Symbole du gène: FANCL
• Identification du gène (NCBI): 55120
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fanconi anemia (FA) is a hereditary bone marrow failure syndrome associated with developmental defects and cancer predisposition. The FA nuclear core complex consists of FANCA, B, C, E, F, G, L, and M. FANCL is a RING-type E3 ubiquitin ligase that monoubiquitinates FANCD2 and FANCI, a key functional role facilitated by other members of the nuclear core complex (PMID: 23783032). FANCL plays an important role in the repair of cisplatin-induced DNA damage (PMID: 26385482) and participates directly in support of stem cell function (PMID: 23783032). FANCL can be detected as 40-43 kDa (full-length) and ~28 kDa.

Publications

Species	Application	Title
	WB	Nat Struct Mol Biol; Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.; Authors - Xinlin Xu ; KO Validated
human	WB	J Mol Biol; Mitotic DNA synthesis in untransformed human cells preserves common fragile site stability via a FANCD2-driven mechanism that requires HELQ; Authors - Emma L Traband ; KO Validated
human	WB	Cell Rep; FANCD2-dependent mitotic DNA synthesis relies on PCNA K164 ubiquitination; Authors - Wendy Leung ; KO Validated
mouse,human	WB	Nat Commun; Histone-methyltransferase KMT2D deficiency impairs the Fanconi anemia/BRCA pathway upon glycolytic inhibition in squamous cell carcinoma; Authors - Wei Liu

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