Anticorps Recombinant de lapin anti-FAP

FAP Recombinant Antibody for WB, FC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

241129B4

N° de cat : 84018-4-RR

Synonymes

FAPA, 170 kDa melanoma membrane-bound gelatinase, 241129B4, Antiplasmin-cleaving enzyme FAP, soluble form, Dipeptidyl peptidase FAP

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Galerie de données de validation

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  WB analysis of U-87 MG using 84018-4-RR

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 84018-4-RR (FAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected HEK-293 using 84018-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using FAP antibody (84018-4-RR, Clone: 241129B4 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  FC experiment of Transfected HEK-293T using 84018-4-RR

  1x10^6 HEK-293T cells or FAP-transfected HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone: 241129B4) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 84018-4-RR

  1x10^6 untransfected (left) or FAP transfected (right) HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone:241129B4) (red) or Isotype Control (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). 1x10^6 HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone:241129B4) (red), or Isotype Control (blue) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

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  Affinity and Kinetic Characterization of 84018-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84018-4-RR against Human FAP were performed. The affinity constant is 0.126 nM.

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Applications testées

• Résultats positifs en WB: cellules MG U-87,
• Résultats positifs en cytométrie: Transfected HEK-293T cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

84018-4-RR cible FAP dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FAP Protéine recombinante Eg0821
• Nom complet: fibroblast activation protein, alpha
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 88 kDa
• Numéro d’acquisition GenBank: NM_004460.4
• Symbole du gène: FAP
• Identification du gène (NCBI): 2191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).

Publications

Species	Application	Title
mouse	IF	Signal Transduct Target Ther; Photodynamic gel-bombs enhance tumor penetration and downstream synergistic therapies; Authors - Xiaole Bai

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