Anticorps Polyclonal de lapin anti-FAP

FAP Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11779-1-PBS

Synonymes

FAPA, 170 kDa melanoma membrane-bound gelatinase, Antiplasmin-cleaving enzyme FAP, soluble form, Dipeptidyl peptidase FAP, EC:3.4.14.5

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Galerie de données de validation

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  WB analysis using 11779-1-AP (same clone as 11779-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11779-1-AP (FAPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11779-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 11779-1-AP (same clone as 11779-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11779-1-AP (FAPA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11779-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11779-1-PBS cible FAP dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAP Protéine recombinante Ag2439
• Nom complet: fibroblast activation protein, alpha
• Masse moléculaire calculée: 760 aa, 88 kDa
• Poids moléculaire observé: 87-97 kDa
• Numéro d’acquisition GenBank: BC026250
• Symbole du gène: FAP
• Identification du gène (NCBI): 2191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).

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