Anticorps Polyclonal de lapin anti-FARSB

FARSB Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16341-1-AP

Synonymes

FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS

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Galerie de données de validation

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  WB analysis using 16341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16341-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 16341-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16341-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16341-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16341-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16341-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16341-1-AP

  IP Result of anti-FARSB (IP:16341-1-AP, 4ug; Detection:16341-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16341-1-AP (FARSB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 16341-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16341-1-AP (FARSB antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16341-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16341-1-AP (FARSB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules HL-60, cellules K-562, cellules PC-3, tissu cérébral de souris, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

16341-1-AP cible FARSB dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FARSB Protéine recombinante Ag9522
• Nom complet: phenylalanyl-tRNA synthetase, beta subunit
• Masse moléculaire calculée: 589 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC017783
• Symbole du gène: FARSB
• Identification du gène (NCBI): 10056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,rat	WB	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji
rat	WB	Neural Regen Res; A novel therapeutic target for peripheral nerve injury-related diseases: aminoacyl-tRNA synthetases.; Authors - Byung Sun Park
human,mouse	WB,IF	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj
human,mouse	WB,IHC,IF	Int J Mol Sci; FARSB Facilitates Hepatocellular Carcinoma Progression by Activating the mTORC1 Signaling Pathway; Authors - Yaofeng Wang