Anticorps Monoclonal anti-FARSB

• Phare
• Validé par KD/KO

FARSB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H10C2

N° de cat : 67924-1-PBS

Synonymes

FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS

---

Galerie de données de validation

• 

  WB analysis using 67924-1-Ig (same clone as 67924-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67924-1-Ig (same clone as 67924-1-PBS)

  WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HepG2 cells. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67924-1-Ig (same clone as 67924-1-PBS)

  WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HeLa cells. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67924-1-Ig (same clone as 67924-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67924-1-Ig (same clone as 67924-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67924-1-Ig (same clone as 67924-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 67924-1-Ig (same clone as 67924-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67924-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67924-1-PBS cible FARSB dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FARSB Protéine recombinante Ag9695
• Nom complet: phenylalanyl-tRNA synthetase, beta subunit
• Masse moléculaire calculée: 589 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC017783
• Symbole du gène: FARSB
• Identification du gène (NCBI): 10056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}