Anticorps Polyclonal de lapin anti-FAS/CD95

• Phare
• Validé par KD/KO

FAS/CD95 Polyclonal Antibody for FC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, FC, CoIP, CHIP, ELISA

Conjugaison

Non conjugué

Publications

111

Avis (2)

N° de cat : 13098-1-AP

Synonymes

ALPS1A, APO 1, Apo 1 antigen, APT1, CD95, FAS, FAS/CD95, FAS1, FASLG receptor, FASTM, TNFRSF6

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Galerie de données de validation

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  WB analysis of Jurkat using 13098-1-AP

  WB result of FAS/CD95 antibody (13098-1-AP, 1:1000) with si-control and si-FAS transfected Jurkat cells.

• 

  WB analysis using 13098-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13098-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13098-1-AP

  IP Result of anti-FAS/CD95 (IP:13098-1-AP, 3ug; Detection:13098-1-AP 1:500) with HeLa cells lysate 3000ug.

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  FC experiment of Jurkat using 13098-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug FAS/CD95 antibody (13098-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules HeLa, cellules HT-29, cellules Jurkat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 102 publications below
• IHC: See 10 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

13098-1-AP cible FAS/CD95 dans les applications de WB, IP, IHC, IF, FC, CoIP, CHIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, singe, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FAS/CD95 Protéine recombinante Ag3718
• Nom complet: Fas (TNF receptor superfamily, member 6)
• Masse moléculaire calculée: 35-38 kDa
• Poids moléculaire observé: 38-45 kDa
• Numéro d’acquisition GenBank: BC012479
• Symbole du gène: FAS
• Identification du gène (NCBI): 355
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.

Publications

Species	Application	Title
human	IF	Cell; Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.; Authors - Chang Cui
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Clin Transl Med; c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis.; Authors - Lan Zhang
	WB	Phytomedicine; Garlic-derived exosomes regulate PFKFB3 expression to relieve liver dysfunction in high-fat diet-fed mice via macrophage-hepatocyte crosstalk; Authors - Jinfan Liu
		Diabetes; PMID: 23990362; Authors - Wang Qun Q
	WB,IHC	Cancer Lett; Fas signaling in adipocytes promotes low-grade inflammation and lung metastasis of colorectal cancer through interaction with Bmx.; Authors - Fei Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena Lucia (Verified Customer) (11-16-2021)

Some unspecific binding but the target is very easy to identify.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ovarian ca cell lines / melanoma cell lines

FH

Kishor (Verified Customer) (06-23-2019)

This antibody works good at 1:1000 dilution in WB for mouse and human cells.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human and mouse cell lines

FAS/CD95 Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in Human and mouse cell lines (Cat no:13098-1-AP)

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