- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-FASN
FASN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (6)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Conjugaison
Non conjugué
N° de cat : 10624-2-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HEK-293, cellules HeLa, cellules HepG2, cellules SH-SY5Y, tissu hépatique de rat, tissu hépatique de souris |
| Résultats positifs en IP | tissu hépatique de souris, |
| Résultats positifs en IHC | tissu de cancer du sein humain, tissu adipeux brun de souris, tissu cérébral de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF/ICC | cellules HeLa, |
| Résultats positifs en FC (Intra) | cellules HeLa |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochimie (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 5 publications below |
| WB | See 255 publications below |
| IHC | See 36 publications below |
| IF | See 14 publications below |
| IP | See 5 publications below |
| CoIP | See 1 publications below |
Informations sur le produit
10624-2-AP cible FASN dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, bovin, Chèvre, Humain, poisson-zèbre, porc, poulet, souris, duck |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | FASN Protéine recombinante Ag0975 |
| Nom complet | fatty acid synthase |
| Masse moléculaire calculée | 272 kDa |
| Poids moléculaire observé | 250-272 kDa |
| Numéro d’acquisition GenBank | BC007909 |
| Symbole du gène | FASN |
| Identification du gène (NCBI) | 2194 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for FASN antibody 10624-2-AP | Download protocol |
| IHC protocol for FASN antibody 10624-2-AP | Download protocol |
| IF protocol for FASN antibody 10624-2-AP | Download protocol |
| IP protocol for FASN antibody 10624-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate | ||
Nat Cancer Integrative proteogenomic profiling of high-risk prostate cancer samples from Chinese patients indicates metabolic vulnerabilities and diagnostic biomarkers | ||
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. | ||
Nat Commun A nanoemulsion targeting adipose hypertrophy and hyperplasia shows anti-obesity efficiency in female mice | ||
Nat Commun Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH YINGJIAN (Verified Customer) (08-07-2025) | Clear signal and very low background—saved a lot of optimization time
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FH K (Verified Customer) (12-20-2020) | I got good results with this Ab for WB at (1:1000) and IHC (1:200)
|
FH Iram (Verified Customer) (09-18-2020) | FASN antibody gives a very clean bands.Excellent antibody
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