Anticorps Recombinant de lapin anti-FAT1

FAT1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241602F9

N° de cat : 84279-3-RR

Synonymes

241602F9, CDHF7, FAT, hFat1, ME5

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Galerie de données de validation

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  WB analysis of HeLa using 84279-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 84279-3-RR (FAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84279-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84279-3-RR against Human FAT1 were performed. The affinity constant is 0.375 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84279-3-RR cible FAT1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FAT1 Protéine recombinante Ag33957
• Nom complet: FAT tumor suppressor homolog 1 (Drosophila)
• Poids moléculaire observé: 506-600 kDa, 85 kDa
• Symbole du gène: FAT1
• Identification du gène (NCBI): 2195
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FAT1, also known as hFat1, belongs to a member of the cadherin superfamily, has been proposed to play roles in cerebral development, glomerular slit formation, and also to act as a tumor suppressor, but its mechanisms of action have not been elucidated. It is expected to be located in cell membrane and nucleus, which is expressed in many epithelial and some endothelial and smooth muscle cells. The calculated molecular weight of FAT1 is 506 kDa and there is glycosylation modification of the protein. To examine functions of the transmembrane and cytoplasmic domains, they were expressed in HEK-293 and HeLa cells as chimeric proteins in fusion with EGFP and extracellular domains derived from E-cadherin. Proteins comprising the transmembrane domain localized to the membrane fraction (PMID: 15922730, 26373379). The high molecular mass band observed at the expected 500-kDa size of FAT1 is indicated by an arrow together with a prominent band at 85 kDa (PMID: 21680732).

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