Anticorps Polyclonal de lapin anti-FATS

FATS Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30190-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 30190-1-AP (same clone as 30190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30190-1-AP (FATS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30190-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 30190-1-AP (same clone as 30190-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FATS antibody (30190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30190-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30190-1-PBS cible FATS dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FATS Protéine recombinante Ag32956
• Nom complet: chromosome 10 open reading frame 90
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 67-77 kDa
• Numéro d’acquisition GenBank: NM_001004298.2
• Symbole du gène: FATS/C10orf90
• Identification du gène (NCBI): 118611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FATS, also known as centrosomal protein C10orf90, bA422P15.2, belongs to a novel class of enzymes that acts as an E2-E3 hybrid enzyme for assembly of polyubiquitin chains. FATS is an E2-independent ubiquitin ligase that stabilizes p53 and promotes its activation in response to DNA damage (PMID: 24240685). FATS acts as a p53/TP53 activator by inhibiting MDM2 binding to p53/TP53 and stimulating non-proteolytic polyubiquitination of p53/TP53.

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