• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-FBL

FBL Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(35)

N° de cat : 16021-1-AP

Synonymes

EC:2.1.1.-, FIB, FIB1, fibrillarin, FLRN



Applications testées

Résultats positifs en WBcellules A375, tissu hépatique de souris
Résultats positifs en IPcellules A375
Résultats positifs en IF/ICCcellules HepG2,
Résultats positifs en FC (Intra)cellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

16021-1-AP cible FBL dans les applications de WB, IF/ICC, FC (Intra), IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène FBL Protéine recombinante Ag8841
Nom complet fibrillarin
Masse moléculaire calculée 321 aa, 34 kDa
Poids moléculaire observé 34 kDa
Numéro d’acquisition GenBankBC019260
Symbole du gène FBL
Identification du gène (NCBI) 2091
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Protocole

Product Specific Protocols
WB protocol for FBL antibody 16021-1-APDownload protocol
IHC protocol for FBL antibody 16021-1-APDownload protocol
IF protocol for FBL antibody 16021-1-APDownload protocol
IP protocol for FBL antibody 16021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Oxidative cyclization reagents reveal tryptophan cation-π interactions

Authors - Xiao Xie
humanWB,RIP,IF

Mol Cell

lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing

Authors - Yu-Meng Sun
humanWB

Mol Cell

LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.

Authors - Xin Wang
  • KD Validated
humanWB

Nucleic Acids Res

GPATCH4 functions as a regulator of nucleolar R-loops in hepatocellular carcinoma cells

Authors - Yi-Ming Zhao
humanWB

Breast Cancer Res

U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression

Authors - Wenrong Zhang
humanWB,IF

Cell Biol Toxicol

SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells

Authors - Jingyi Cui

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Tatyana (Verified Customer) (03-11-2023)

Very strong, specific nucleolar signal in ICC. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h RT incubation (no blocking step).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human, U2OS cells
FH

WEI (Verified Customer) (03-12-2020)

Good, strong band for WB and correct staining for cardiomyocytes.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Mouse Heart
FH

Lauren (Verified Customer) (02-20-2020)

Fixations tested include 2% and 4% formaldehyde for 20 minutes, and -20'C methanol for 10 minutes. Antibody works in all three fixations, but 4% formaldehyde gave the cleanest signal.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mIMCD3
FBL Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:16021-1-AP)
FH

Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human satelllite muscle cells
FBL Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:16021-1-AP)
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