Anticorps Polyclonal de lapin anti-FBL

• Phare
• Validé par KD/KO

FBL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (4)

N° de cat : 16021-1-AP

Synonymes

FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1

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Galerie de données de validation

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  WB analysis using 16021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16021-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16021-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 16021-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 16021-1-AP

  IP Result of anti-FBL (IP:16021-1-AP, 4ug; Detection:16021-1-AP 1:500) with A375 cells lysate 3600ug.

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  IHC staining of mouse brain using 16021-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16021-1-AP (FBL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16021-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16021-1-AP (FBL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16021-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 16021-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 16021-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A375, tissu hépatique de souris
• Résultats positifs en IP: cellules A375
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• RIP: See 3 publications below

Informations sur le produit

16021-1-AP cible FBL dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBL Protéine recombinante Ag8841
• Nom complet: fibrillarin
• Masse moléculaire calculée: 321 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC019260
• Symbole du gène: FBL
• Identification du gène (NCBI): 2091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.; Authors - Xin Wang ; KD Validated
human	WB,IF	Cell Biol Toxicol; SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells; Authors - Jingyi Cui
	IF	Commun Biol; Nascent ribosomal RNA act as surfactant that suppresses growth of fibrillar centers in nucleolus; Authors - Tetsuya Yamamoto
human	WB	Oncotarget; Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis.; Authors - Xing Liu
human,mouse	WB	Front Immunol; RNA 2'-O-Methyltransferase Fibrillarin Facilitates Virus Entry Into Macrophages Through Inhibiting Type I Interferon Response.; Authors - Panpan Li ; KD Validated
human	RIP	Mol Carcinog; SNORD60 promotes the tumorigenesis and progression of endometrial cancer through binding PIK3CA and regulating PI3K/AKT/mTOR signaling pathway; Authors - Wu Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

Very strong, specific nucleolar signal in ICC. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h RT incubation (no blocking step).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, U2OS cells

FH

WEI (Verified Customer) (03-12-2020)

Good, strong band for WB and correct staining for cardiomyocytes.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Mouse Heart

FH

Lauren (Verified Customer) (02-20-2020)

Fixations tested include 2% and 4% formaldehyde for 20 minutes, and -20'C methanol for 10 minutes. Antibody works in all three fixations, but 4% formaldehyde gave the cleanest signal.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mIMCD3

FBL Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:16021-1-AP)

FH

Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human satelllite muscle cells

FBL Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:16021-1-AP)