Anticorps Monoclonal anti-FBL

FBL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

3A9E8

N° de cat : 66985-1-Ig

Synonymes

FLRN, fibrillarin, FIB1, FIB, EC:2.1.1.-

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Galerie de données de validation

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  WB analysis using 66985-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66985-1-Ig (FBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 66985-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66985-1-Ig (FBL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 66985-1-Ig

  IP result of anti-FBL (IP:66985-1-Ig, 5ug; Detection:66985-1-Ig 1:1000) with HeLa cells lysate 1600 ug.

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  IHC staining of human liver cancer using 66985-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66985-1-Ig (FBL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66985-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66985-1-Ig (FBL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66985-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

• 

  IF Staining of HeLa using 66985-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

• 

  IF Staining of HepG2 using 66985-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66985-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FBL (66985-1-Ig, Clone:3A9E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of HeLa using 66985-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug FBL Monoclonal antibody (66985-1-Ig, Clone:3A9E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 5 publications below
• RIP: See 1 publications below

Informations sur le produit

66985-1-Ig cible FBL dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FBL Protéine recombinante Ag8841
• Nom complet: fibrillarin
• Masse moléculaire calculée: 321 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC019260
• Symbole du gène: FBL
• Identification du gène (NCBI): 2091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).

Publications

Species	Application	Title
human	WB,IF	Gastroenterology; Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis; Authors - Xiao-Mei Yang
human	WB,RIP,IF	Mol Cell; lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing; Authors - Yu-Meng Sun
human	WB	BMC Biol; A pair of long intergenic non-coding RNA LINC00887 variants act antagonistically to control Carbonic Anhydrase IX transcription upon hypoxia in tongue squamous carcinoma progression; Authors - Tao Shen
human	WB	Thorac Cancer; LncRNA FAM83A-AS1 promotes lung adenocarcinoma progression by enhancing the pre-mRNA stability of FAM83A.; Authors - Wenyi Wang
human	IF	Cell Death Discov; Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage; Authors - Jingtao Liu
human	WB,IF	Nat Commun; NAP-seq reveals multiple classes of structured noncoding RNAs with regulatory functions; Authors - Shurong Liu

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