Anticorps Monoclonal anti-FBP1

FBP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G4E4

N° de cat : 68446-1-PBS

Synonymes

FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1

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Galerie de données de validation

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  WB analysis using 68446-1-Ig (same clone as 68446-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68446-1-Ig (same clone as 68446-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 68446-1-Ig (same clone as 68446-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 68446-1-Ig (same clone as 68446-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68446-1-Ig (same clone as 68446-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FBP1 antibody (68446-1-Ig, Clone: 2G4E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68446-1-PBS cible FBP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FBP1 Protéine recombinante Ag3837
• Nom complet: fructose-1,6-bisphosphatase 1
• Masse moléculaire calculée: 338 aa, 37 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC012927
• Symbole du gène: FBP1
• Identification du gène (NCBI): 2203
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).

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