Anticorps Polyclonal de lapin anti-FBP17

FBP17 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (2)

N° de cat : 27056-1-AP

Synonymes

FBP17, FNBP1, formin binding protein 1, Formin binding protein 17, hFBP17, KIAA0554

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Galerie de données de validation

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  WB analysis of mouse brain using 27056-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27056-1-AP (FBP17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 27056-1-AP

  IP result of anti-FBP17 (IP:27056-1-AP, 4ug; Detection:27056-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

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  IHC staining of human lung cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cardiaque de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:150-1:600
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

27056-1-AP cible FBP17 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBP17 Protéine recombinante Ag25784
• Nom complet: formin binding protein 1
• Poids moléculaire observé: 80-85 kDa
• Numéro d’acquisition GenBank: BC101755
• Symbole du gène: FBP17
• Identification du gène (NCBI): 23048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBP17 (formin-binding protein 17) is a new dynamin-associating molecule consisting of several functional domains, including an N-terminal EFC domain and an SH3 domain at the C-terminus. FBP17 is involved in dynamin-mediated endocytosis and has been reported to be required for invadopodia formation and tumor cell invasion.

Publications

Species	Application	Title
human	WB	bioRxiv; F-BAR proteins CIP4 and FBP17 function in cortical neuron radial migration and process outgrowth; Authors - Lauren A English

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cicek (Verified Customer) (06-11-2024)

I tested the antibody on mouse neurons, iPSCs and 3-weeks-old iNeurons with Western blotting using beta-actin as positive control. There were bands at the 80 kDa mark showing FBP17 for iPSC and iNeurons, but not mouse neurons. All samples showed a strong unspecific band at 70 kDa mark.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

María (Verified Customer) (02-08-2022)

Works fine for WB (1:1000), for IF some background, even after several washes with detergent

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela/RPE-1/MEF/NaTi

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