Anticorps Recombinant de lapin anti-FBP2

FBP2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240879C9

N° de cat : 83910-5-RR

Synonymes

Muscle FBPase, Fructose-1,6-bisphosphatase isozyme 2, FBPase 2, EC:3.1.3.11, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2

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Galerie de données de validation

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  WB analysis of mouse heart using 83910-5-RR

  Mouse heart tissue were subjected to SDS PAGE followed by western blot with 83910-5-RR (FBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 83910-5-RR

  Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 83910-5-RR (FBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 83910-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 83910-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 83910-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 83910-5-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBP2 antibody (83910-5-RR, Clone: 240879C9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83910-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83910-5-RR against Human FBP2 were performed. The affinity constant is 4.67 nM.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu de muscle squelettique de rat
• Résultats positifs en IHC: tissu de muscle squelettique de souris, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: tissu de muscle squelettique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83910-5-RR cible FBP2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FBP2 Protéine recombinante Ag18245
• Nom complet: fructose-1,6-bisphosphatase 2
• Masse moléculaire calculée: 339 aa, 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC113632
• Symbole du gène: FBP2
• Identification du gène (NCBI): 8789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBP2, also named muscle FBP, belongs to the FBPase class 1 family. FBP2 is a ubiquitously expressed enzyme of glycogen synthesis from non-carbohydrates, e.g., lactate (glyconeogenesis). However, it also plays a variety of non-enzymatic functions. FBP2 is involved in the regulation of hypoxia-inducible factor 1 (Hif1) stability, cell cycle-dependent events, biogenesis of mitochondria, and protection of the organelles against high reactive oxygen species (ROS)- and high Ca2+-induced stress (PMID: 32962293, PMID: 35626746). The calculated molecular weight of FBP2 is 37 kDa.

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