Anticorps Polyclonal de lapin anti-FBX15

• Phare
• Validé par KD/KO

FBX15 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13024-1-PBS

Synonymes

FBXO15, F-box only protein 15, F box protein 15, F box only protein 15

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Galerie de données de validation

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  WB analysis of A2780 using 13024-1-AP (same clone as 13024-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 13024-1-AP (FBX15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13024-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 13024-1-AP (same clone as 13024-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13024-1-AP (FBX15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13024-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 13024-1-AP (same clone as 13024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13024-1-AP (FBX15 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13024-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13024-1-PBS cible FBX15 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBX15 Protéine recombinante Ag3564
• Nom complet: F-box protein 15
• Masse moléculaire calculée: 434 aa, 49 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC029579
• Symbole du gène: FBX15
• Identification du gène (NCBI): 201456
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. The F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. FBOX15 (or FBX15) belongs to the FBXs class, and 2 isoforms produced by alternative splicing has been described. This antibody is expected detect both isoforms.

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