Anticorps Polyclonal de lapin anti-FBXO17

• Phare
• Validé par KD/KO

FBXO17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 12844-1-AP

Synonymes

FBG4, F-box only protein 17, F-box only protein 26, FBX17, FBX26

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Galerie de données de validation

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  WB analysis using 12844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12844-1-AP (FBXO17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12844-1-AP

  WB result of FBXO17 antibody (12844-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXO17 transfected HepG2 cells.

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  WB analysis of mouse heart using 12844-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12844-1-AP (FBXO17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 12844-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FBXO17 antibody (12844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 12844-1-AP

  Immunofluorescent analysis of HeLa cells using 12844-1-AP (FBXO17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules HepG2, tissu cardiaque de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below

Informations sur le produit

12844-1-AP cible FBXO17 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBXO17 Protéine recombinante Ag3867
• Nom complet: F-box protein 17
• Masse moléculaire calculée: 278 aa, 31 kDa
• Poids moléculaire observé: 29-31 kDa
• Numéro d’acquisition GenBank: BC012385
• Symbole du gène: FBXO17
• Identification du gène (NCBI): 115290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu
mouse	WB	J Biol Chem; SCFFBXO17 E3 ligase modulates inflammation by regulating proteasomal degradation of glycogen synthase kinase-3β in lung epithelia.; Authors - Tomeka Suber
human	IHC	Carcinogenesis; Systematically characterize the clinical and biological significances of 1p19q genes in 1p/19q non-codeletion glioma.; Authors - Rui-Chao Chai
human	WB,IHC	J Gene Med; Circ_0008717 promotes renal cell carcinoma progression by upregulating FBXO17 via targeting miR-217.; Authors - Siyao Shen
human	WB	Biosci Biotechnol Biochem; F-box protein 17 promotes glioma progression by regulating glycolysis pathway.; Authors - Chao Deng ; KD Validated
mouse,human	WB,IHC	Int J Med Sci; FBXO17 Inhibits the Wnt/β-Catenin Pathway and Proliferation of Ishikawa Cells; Authors - Zi-Meng Zheng

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