Anticorps Polyclonal de lapin anti-FBXO2

• Phare
• Validé par KD/KO

FBXO2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 14590-1-AP

Synonymes

FBG1, F-box only protein 2, Fbs1, FBX2, NFB42

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Galerie de données de validation

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  WB analysis using 14590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14590-1-AP (FBXO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14590-1-AP

  SH-SY5Y lysates were subjected to SDS PAGE followed by western blot with 14590-1-AP (FBXO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14590-1-AP (FBXO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14590-1-AP (FBXO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of rat brain using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat cerebellum using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of rat cerebellum using 14590-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum using 14590-1-AP (FBXO2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules Caco-2, cellules HCT 116
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu de cervelet de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

14590-1-AP cible FBXO2 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBXO2 Protéine recombinante Ag6122
• Nom complet: F-box protein 2
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33-40 kDa
• Numéro d’acquisition GenBank: BC025233
• Symbole du gène: FBXO2
• Identification du gène (NCBI): 26232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

F-box only protein 2 (FBXO2), also known as FBG1 or Fbs1, is a cytoplasmic protein and ubiquitin ligase with high-mannose glycoprotein specificity. FBXO2 plays a significant role in regulating normal and abnormal neuron functions as a ubiquitin-mediated degrader of several neuron glycoproteins. In addition, it has been reported that FBXO2 targets insulin receptors for degradation by ubiquitin, thereby modulating insulin signaling. Recently, emerging evidence demonstrated that FBXO2 was also strongly associated with the occurrence and development of malignant tumors, including gastric cancer and colorectal cancer. The overexpression of FBXO2 significantly promotes PTC cell proliferation (PMID: 39343799). Lactic acid mediates the selective loading of FBXO2 into SEVs through SORBS3/FLOT1, thereby inducing hepatocyte apoptosis, which is an important mechanism of liver fibrosis caused by myogenic SEVs (PMID: 39879982).

Publications

Species	Application	Title
human	WB	Sci Rep; Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues.; Authors - Yanyan Gao
human	WB,IHC	Biochem Biophys Res Commun; FBXO2, a novel marker for metastasis in human gastric cancer.; Authors - Sun Xu
human	WB	Front Med (Lausanne); Follicular dendritic cell differentiation is associated with distinct synovial pathotype signatures in rheumatoid arthritis; Authors - Mohey Eldin M El Shikh ; KD Validated
mouse	WB,IHC,CoIP	Nat Commun; Inhibition of histone methyltransferase Smyd3 rescues NMDAR and cognitive deficits in a tauopathy mouse model; Authors - Jamal B Williams
mouse,human	WB,IP,IHC,IF	World J Gastroenterol; F-box only protein 2 exacerbates non-alcoholic fatty liver disease by targeting the hydroxyl CoA dehydrogenase alpha subunit; Authors - Zhi Liu ; KD Validated
mouse	IF	Stem Cells; Early Wnt Signaling Activation Promotes Inner Ear Differentiation via Cell Caudalization in Mouse Stem Cell-Derived Organoids; Authors - Pei-Ciao Tang

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