Anticorps Polyclonal de lapin anti-FBXO22

• Phare
• Validé par KD/KO

FBXO22 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (1)

N° de cat : 13606-1-AP

Synonymes

F box only protein 22, F box protein 22, F box protein FBX22p44, FBX22, FBXO22, FISTC1

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Galerie de données de validation

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  WB analysis using 13606-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13606-1-AP (FBXO22 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 13606-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 13606-1-AP (FBXO22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13606-1-AP

  IP Result of anti-FBXO22 (IP:13606-1-AP, 4ug; Detection:13606-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of mouse heart using 13606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13606-1-AP (FBXO22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13606-1-AP (FBXO22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HuH-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 19 publications below
• IHC: See 8 publications below
• IP: See 1 publications below

Informations sur le produit

13606-1-AP cible FBXO22 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBXO22 Protéine recombinante Ag4485
• Nom complet: F-box protein 22
• Masse moléculaire calculée: 403 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC039024
• Symbole du gène: FBXO22
• Identification du gène (NCBI): 26263
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBXO22, also named as FBX22, is a substrate-recognition of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. FBXO22 is a key regulator of senescence induction through ubiquitylation of p53 for degradation. FBXO22 also acts as a molecular switch for the antagonistic and agonistic actions of selective estrogen receptor modulators (SERM) and determines the sensitivity of breast cancer to SERM by ubiquitylating KDM4B complexed with unliganded or SERMs‐bound estrogen receptor (ER). (PMID: 32536008, PMID: 32249768)

Publications

Species	Application	Title
human	WB,IHC	Cell; Nrf2 Activation Promotes Lung Cancer Metastasis by Inhibiting the Degradation of Bach1.; Authors - Luca Lignitto ; KO Validated
human	WB	Nat Commun; Targeted inhibition of the COP9 signalosome for treatment of cancer.; Authors - Anita Schlierf
human	WB	Cell Death Differ; Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.; Authors - Ping Liu ; KO Validated
human	WB	Front Oncol; Pan-Cancer Analyses Reveal Oncogenic Role and Prognostic Value of F-Box Only Protein 22.; Authors - Sen Chen
human	WB	iScience; A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.; Authors - Muzammal Hussain
human	WB,IHC	Front Pharmacol; FBXO22 Promotes Growth and Metastasis and Inhibits Autophagy in Epithelial Ovarian Cancers via the MAPK/ERK Pathway.; Authors - Minle Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Strong single band at predicted size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart