Anticorps Monoclonal anti-FBXO32

FBXO32 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

23

CloneNo.

3C2G3

N° de cat : 67172-1-Ig

Synonymes

Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein

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Galerie de données de validation

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  WB analysis using 67172-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 67172-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67172-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skeletal muscle using 67172-1-Ig

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 67172-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 67172-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 67172-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 67172-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 67172-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 67172-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cardiaque de porc, tissu cardiaque humain, tissu de muscle squelettique de lapin, tissu de muscle squelettique de porc
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu de muscle squelettique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de muscle squelettique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:10000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IHC: See 10 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

67172-1-Ig cible FBXO32 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FBXO32 Protéine recombinante Ag25247
• Nom complet: F-box protein 32
• Masse moléculaire calculée: 355 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC024030
• Symbole du gène: FBXO32
• Identification du gène (NCBI): 114907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.

Publications

Species	Application	Title
mouse	WB	Cell Biol Toxicol; Statins induce skeletal muscle atrophy via GGPP depletion-dependent myostatin overexpression in skeletal muscle and brown adipose tissue.; Authors - Lai Wang
pig	WB	J Anim Sci Biotechnol; Protective effects of taurine against muscle damage induced by diquat in 35 days weaned piglets.; Authors - Chaoyue Wen
canine	WB,IHC	Life Sci; N-acetylcysteine alleviates oxidative stress and apoptosis and prevents skeletal muscle atrophy in type 1 diabetes mellitus through the NRF2/HO-1 pathway; Authors - Qingyu Ding
mouse	WB	J Ethnopharmacol; Saponin extract from Achyranthes bidentata Blume alleviates disuse-induced muscle atrophy through PI3K/Akt signaling pathway; Authors - Yi Shi
mouse	WB	Front Cell Dev Biol; Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia.; Authors - Xiangyu Mao
mouse	WB	Exp Cell Res; Resistance exercise alleviates dexamethasone-induced muscle atrophy via Sestrin2/MSTN pathway in C57BL/6J mice; Authors - Yang Yang