Anticorps Monoclonal anti-FBXO32

FBXO32 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C2G3

N° de cat : 67172-1-PBS

Synonymes

3C2G3, Atrogin 1, Atrogin-1, F box only protein 32, F box protein 32

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Galerie de données de validation

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  WB analysis using 67172-1-Ig (same clone as 67172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 67172-1-Ig (same clone as 67172-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 67172-1-Ig (same clone as 67172-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

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  WB analysis of pig skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67172-1-PBS cible FBXO32 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FBXO32 Protéine recombinante Ag25247
• Nom complet: F-box protein 32
• Masse moléculaire calculée: 355 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC024030
• Symbole du gène: FBXO32
• Identification du gène (NCBI): 114907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.

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