Anticorps Polyclonal de lapin anti-FBXO33

FBXO33 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23381-1-PBS

Synonymes

FBX33, F-box only protein 33, c14_5247, BMND12

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Galerie de données de validation

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  WB analysis using 23381-1-AP (same clone as 23381-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23381-1-AP (FBXO33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23381-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 23381-1-AP (same clone as 23381-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FBXO33 antibody (23381-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23381-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23381-1-PBS cible FBXO33 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBXO33 Protéine recombinante Ag20055
• Nom complet: F-box protein 33
• Masse moléculaire calculée: 555 aa, 63 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC068566
• Symbole du gène: FBXO33
• Identification du gène (NCBI): 254170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

F-box Only Protein 33 (FBXO33) is a typical member of the F-box protein family with E3 ligase function. FBXO33 can regulate the ubiquitination and solubility of polyglutamine disease proteins, potentially serving as a target for treating type 3 spinocerebellar ataxia. FBXO33 ligase interferes with YB-1-mediated functions by binding and ubiquitinating the multifunctional regulator Y-box-binding protein 1 (YB-1)/dbpB/p50, leading to proteasomal degradation (PMID: 39206900). The calculated molecular weight of FBXO33 is 63 kDa.

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