Anticorps Recombinant de lapin anti-FBXO38

• Phare
• Validé par KD/KO

FBXO38 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240513C9

N° de cat : 83509-6-RR

Synonymes

SP329, MOKA, Fbx38, F box protein 38, F box only protein 38

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Galerie de données de validation

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  WB analysis of HeLa using 83509-6-RR

  WB result of FBXO38 antibody (83509-6-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXO38 transfected HeLa cells.

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  WB analysis using 83509-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83509-6-RR (FBXO38 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83509-6-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FBXO38 Recombinant antibody (83509-6-RR, Clone:240513C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83509-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83509-6-RR against Human FBXO38 were performed. The affinity constant is 0.723 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

83509-6-RR cible FBXO38 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FBXO38 Protéine recombinante Ag35444
• Nom complet: F-box protein 38
• Masse moléculaire calculée: 134kDa，1188aa
• Poids moléculaire observé: 133 kDa
• Numéro d’acquisition GenBank: XM_005268513.1
• Symbole du gène: FBXO38
• Identification du gène (NCBI): 81545
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBXO38, also known as HMN2D, MOKA, and SP329, is a protein that plays a significant role in the regulation of immune responses, particularly in the context of cancer and autoimmune diseases. FBXO38 has been identified as an E3 ubiquitin ligase that mediates the degradation of PD-1 (Programmed cell death protein 1), a key immune checkpoint protein expressed on the surface of T cells. The internalization, ubiquitination, and proteasome degradation of PD-1 are facilitated by FBXO38, which links PD-1 to Lys48, leading to its poly-ubiquitination and subsequent degradation (PMID: 30487606).

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; Identification and experimental validation of hub genes underlying depressive-like behaviors induced by chronic social defeat stress; Authors - Yexiang Chen

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