Anticorps Polyclonal de lapin anti-FBXO44

FBXO44 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 10626-1-AP

Synonymes

F box only protein 44, F box protein 44, F box protein FBX30, F box/G domain protein 3, FBG3, FBX30, Fbx44, FBX6A, FBXO44, Fbxo6a

---

Galerie de données de validation

• 

  WB analysis using 10626-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10626-1-AP (FBXO44 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 10626-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10626-1-AP (FBXO44 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10626-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10626-1-AP (FBXO44 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below

Informations sur le produit

10626-1-AP cible FBXO44 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBXO44 Protéine recombinante Ag0977
• Nom complet: F-box protein 44
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC007832
• Symbole du gène: FBXO44
• Identification du gène (NCBI): 93611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)

Publications

Species	Application	Title
human,mouse	IHC	Nat Commun; Integrative proteomic characterization of adenocarcinoma of esophagogastric junction; Authors - Shengli Li
human	IHC	Sci Rep; OTX2 expression contributes progression of gastric cancer in young adults; Authors - Jingming Zhai
human	WB	J Control Release; Core-shell vector-mediated co-delivery of CRISPR/Cas9 system and hydrophobic drugs against triple-negative breast cancer stem cells; Authors - Tong Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tongbin (Verified Customer) (08-25-2020)

This antibody had some non-specific bands in western blots. Nevertheless, the specific Fbxo44 band at around 30 kDa can still be seen.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Heart

{{ptg:RelatedPrimaryAntibodies}}