Anticorps Polyclonal de lapin anti-FBXO5

FBXO5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 10872-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 10872-1-AP (same clone as 10872-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10872-1-AP (same clone as 10872-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10872-1-AP (same clone as 10872-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10872-1-AP (same clone as 10872-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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  WB analysis of human ovary using 10872-1-AP (same clone as 10872-1-PBS)

  human ovary tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 10872-1-AP (same clone as 10872-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 10872-1-AP (same clone as 10872-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 10872-1-AP (same clone as 10872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10872-1-PBS cible FBXO5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBXO5 Protéine recombinante Ag1296
• Nom complet: F-box protein 5
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 56 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC018905
• Symbole du gène: FBXO5
• Identification du gène (NCBI): 26271
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.

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