Anticorps Monoclonal anti-FBXO6

• Phare
• Validé par KD/KO

FBXO6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B7F9

N° de cat : 67697-1-PBS

Synonymes

3B7F9, F box only protein 6, F box protein 6, FBG2, F-box only protein 6

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Galerie de données de validation

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  WB analysis using 67697-1-Ig (same clone as 67697-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67697-1-Ig (FBXO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67697-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67697-1-Ig (same clone as 67697-1-PBS)

  WB result of FBXO6 antibody (67697-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXO6 transfected Jurkat cells. This data was developed using the same antibody clone with 67697-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67697-1-Ig (same clone as 67697-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using FBXO6 antibody (67697-1-Ig, Clone: 3B7F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67697-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67697-1-PBS cible FBXO6 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FBXO6 Protéine recombinante Ag30512
• Nom complet: F-box protein 6
• Masse moléculaire calculée: 293 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC020880
• Symbole du gène: FBXO6
• Identification du gène (NCBI): 26270
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FBXO6(F-box only protein 6 ) is also named as FBG2, FBS2, FBX6 and belongs to the F-box protein family. FBXO6 is the substrate recognition component of a Skp1-Cullin1-F-box protein (SCF) ubiquitin E3 ligase complex, recognizing the chitobiose in unfolded N-glycoprotein to target glycoproteins for polyubiquitination and degradation. It can regulate Chk1 ubiquitination and degradation in both normally cycling cells and during replication stress. This protein is mainly detected in the cytoplasm in both cultured cells and in tumor tissues. Its expression is a predictive biomarker of tumor responsiveness to the important anticancer drugs. Positive Fbxo6 expression correlated with early TNM stage and favorable prognosis of NSCLC patients. (PMID:19716789, PMID: 27855403, PMID: 31140586).

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