Anticorps Polyclonal de lapin anti-FBXW11

• Phare
• Validé par KD/KO

FBXW11 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13149-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 13149-1-AP (same clone as 13149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

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  WB analysis using 13149-1-AP (same clone as 13149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 13149-1-AP (same clone as 13149-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

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  WB analysis using 13149-1-AP (same clone as 13149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SGC-7901 using 13149-1-AP (same clone as 13149-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

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  WB analysis of human stomach using 13149-1-AP (same clone as 13149-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13149-1-AP (same clone as 13149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13149-1-AP (same clone as 13149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13149-1-AP (FBXW11 Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13149-1-AP (same clone as 13149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13149-1-AP (FBXW11 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 13149-1-AP (same clone as 13149-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 13149-1-AP (same clone as 13149-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 13149-1-AP (same clone as 13149-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13149-1-PBS cible FBXW11 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBXW11 Protéine recombinante Ag3761
• Nom complet: F-box and WD repeat domain containing 11
• Masse moléculaire calculée: 542 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC026213
• Symbole du gène: FBXW11
• Identification du gène (NCBI): 23291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FBXW11 (also known as HOS or β-TrCP2) is a member of F-box family proteins and plays critical role in regulating the ubiquitination of phosphorylated substrates. Abnormal expression of several FBXW11 is involved in the modulation of various biological events, such as cell cycle, differentiation, migration, inflammation, and apoptosis, through targeting multiple different substrates. For instance, FBXW11 could bind to the phosphorylated IκB and β-catenin, promoting their degradation via the ubiquitin-proteasome system. In addition, FBXW11 expression is markedly increased in mouse skin tumors and promotes tumor growth by activating the NF-κB signaling (PMID: 33640602). FBXW11 has 3 isoforms with the molecular mass of 58-62 kDa.

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