Anticorps Polyclonal de lapin anti-FBXW5

FBXW5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18321-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 18321-1-AP (same clone as 18321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18321-1-AP (FBXW5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 18321-1-AP (same clone as 18321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 18321-1-AP (same clone as 18321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18321-1-AP (same clone as 18321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18321-1-AP (same clone as 18321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18321-1-PBS cible FBXW5 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FBXW5 Protéine recombinante Ag13163
• Nom complet: F-box and WD repeat domain containing 5
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC014297
• Symbole du gène: FBXW5
• Identification du gène (NCBI): 54461
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. FBXW5 (F-box/WD repeat-containing protein 5) is a novel protein containing WD-40 repeats, which were frequently involved in protein-protein interactions. FBXW5 negatively regulates TAK1 in the IL-1beta signaling pathway. By forming different E3 ubiquitin-protein ligase complexes, FBXW5 mediates the polyubiquitination and subsequent degradation of TSC2 and centriolar protein HsSAS-6.

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