Anticorps Polyclonal de lapin anti-FCGR3A/CD16a

FCGR3A/CD16a Polyclonal Antibody for WB, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30356-1-PBS

Synonymes

CD16, Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen

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Galerie de données de validation

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  WB analysis using 30356-1-AP (same clone as 30356-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 30356-1-AP (FCGR3A/CD16a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30356-1-PBS in a different storage buffer formulation.

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  WB analysis using 30356-1-AP (same clone as 30356-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30356-1-AP (FCGR3A/CD16a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30356-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 30356-1-AP (same clone as 30356-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using FCGR3A/CD16a antibody (30356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30356-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30356-1-PBS cible FCGR3A/CD16a dans les applications de WB, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FCGR3A/CD16a Protéine recombinante Ag1281
• Nom complet: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC036723
• Symbole du gène: CD16a
• Identification du gène (NCBI): 2214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3A has a calculated molecular weight of 29 kDa, with an apparent molecular weight of 50-80 kDa due to extensive glycosylation (PMID: 30655272; PMID: 27914599). The antibody is specific for FCGR3A/CD16a.

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