Anticorps Polyclonal de lapin anti-FDXR

FDXR Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15584-1-AP

Synonymes

Adrenodoxin reductase, ADXR, Aerredoxin Reductase, AR, FDXR, Ferredoxin NADP(+) reductase

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Galerie de données de validation

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  WB analysis using 15584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig adrenal gland using 15584-1-AP

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 15584-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15584-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15584-1-AP (FDXR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15584-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15584-1-AP (FDXR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu de glande surrénale de porc, tissu de glande surrénale humain, tissu testiculaire de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

15584-1-AP cible FDXR dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FDXR Protéine recombinante Ag7145
• Nom complet: ferredoxin reductase
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 48-58 kDa
• Numéro d’acquisition GenBank: BC063493
• Symbole du gène: FDXR
• Identification du gène (NCBI): 2232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ferredoxin reductase (FDXR, also known as adrenodoxin reductase) is a mitochondrial membrane-associated flavoprotein. One of its functions is to transfer electrons from NADPH to the two human ferredoxin proteins, FDX1 and FDX2. FDXR is expressed in all tissues with the highest expression in tissues such as the adrenal cortex, that specialize in steroid hormone synthesis. Functionally, FDXR is suggested to be involved in various Functionally, FDXR is suggested to be involved in various. Notably, recent studies have shown that FDXR mutations are associated with mitochondrial disorders, probably due to its role in iron-sulfur cluster protein biosynthesis (PMID: 32304229). FDXR has 7 isoforms with the molecular weight of 48-58 kDa.

Publications

Species	Application	Title
human	WB	J Biol Chem; The neurosteroid pregnenolone is synthesized by a mitochondrial P450 enzyme other than CYP11A1 in human glial cells.; Authors - Yiqi Christina Lin
human	WB	Front Oncol; FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation; Authors - Chaojun Yan ; KD Validated