Anticorps Monoclonal anti-FER

FER Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1E3H2

N° de cat : 67671-1-Ig

Synonymes

FER, p94 Fer, Proto oncogene c Fer, TYK3, Tyrosine kinase 3, Tyrosine protein kinase Fer

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Galerie de données de validation

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  WB analysis using 67671-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of ROS1728 using 67671-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67671-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 67671-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using FER antibody (67671-1-Ig, Clone: 1E3H2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules ROS1728
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67671-1-Ig cible FER dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FER Protéine recombinante Ag19176
• Nom complet: fer (fps/fes related) tyrosine kinase
• Masse moléculaire calculée: 822 aa, 95 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC017060
• Symbole du gène: FER
• Identification du gène (NCBI): 2241
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Rep; THBS2-producing matrix CAFs promote colorectal cancer progression and link to poor prognosis via the CD47-MAPK axis; Authors - Zaoqu Liu

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