Anticorps Monoclonal anti-FER

FER Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E3H2

N° de cat : 67671-1-PBS

Synonymes

FER, p94 Fer, Proto oncogene c Fer, TYK3, Tyrosine kinase 3, Tyrosine protein kinase Fer

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Galerie de données de validation

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  WB analysis using 67671-1-Ig (same clone as 67671-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67671-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 67671-1-Ig (same clone as 67671-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67671-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67671-1-Ig (same clone as 67671-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67671-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 67671-1-Ig (same clone as 67671-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using FER antibody (67671-1-Ig, Clone: 1E3H2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67671-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67671-1-PBS cible FER dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FER Protéine recombinante Ag19176
• Nom complet: fer (fps/fes related) tyrosine kinase
• Masse moléculaire calculée: 822 aa, 95 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC017060
• Symbole du gène: FER
• Identification du gène (NCBI): 2241
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.

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