Anticorps Polyclonal de lapin anti-FFAR2

• Phare
• Validé par KD/KO

FFAR2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc et plus (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(33)

Avis (1)

N° de cat : 19952-1-AP

Synonymes

FFA2, FFA2R, free fatty acid receptor 2, G protein coupled receptor 43, GPCR43

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Galerie de données de validation

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  WB analysis using 19952-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 19952-1-AP (FFAR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 19952-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19952-1-AP (FFAR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 19952-1-AP

  pig liver tissue were subjected to SDS PAGE followed by western blot with 19952-1-AP (FFAR2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules HCT 116, cellules HeLa, tissu hépatique de porc
• Résultats positifs en IHC: tissu splénique humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 28 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

19952-1-AP cible FFAR2 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: free fatty acid receptor 2
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 50 kDa, 37 kDa
• Numéro d’acquisition GenBank: NM_005306
• Symbole du gène: FFAR2
• Identification du gène (NCBI): 2867
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Free fatty acid receptors (FFAR) play significant roles in various physiological processes through interaction with their ligands, fatty acids. Free fatty acid receptor 2 (FFAR2, also known as FFA2 or GPR43) is a receptor for short-chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and intestinal immunity (PMID: 12684041). It has been considered a therapeutic target for metabolic and inflammatory conditions (PMID: 23589301). FFAR2 has a calculated molecular weight of 37 kDa and can be glycosylated. The higher apparent molecular weight of 50 kDa has been reported, probably due to glycosylation (PMID: 31707282; 28131568).

Publications

Species	Application	Title
mouse	WB	Food Chem X; Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.; Authors - Jingyu Fang
rat	WB	Chin Med; The relationship between gut microbiota and susceptibility to type 2 diabetes mellitus in rats; Authors - Yongcheng An
human	WB	Chin Med; Shaoyao Decoction reduced T lymphocyte activation by regulating of intestinal flora and 5-hydroxytryptamine metabolism in ulcerative colitis; Authors - Jianhua Zhen
mouse	WB,IHC	Pharmaceuticals (Basel); Xiao Cheng Qi Decoction, an Ancient Chinese Herbal Mixture, Relieves Loperamide-Induced Slow-Transit Constipation in Mice: An Action Mediated by Gut Microbiota; Authors - Amanguli Tuohongerbieke
rat	WB	Phytomedicine; The beneficial effects of Polygonatum sibiricum Red. superfine powder on metabolic hypertensive rats via gut-derived LPS/TLR4 pathway inhibition; Authors - Jie Su
mouse	WB	Food Funct; EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.; Authors - Jian Fang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christin (Verified Customer) (01-26-2025)

Antibody used to study G protein coupled receptors responsible for uptake of short chain fatty acids in human iPSC-derived microglia and macrophages.

• Applications: Western Blot
• Primary Antibody Dilution: 1:750 in milk
• Cell Tissue Type: Human iPSC-derived macrophages and microglia

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