- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-FFAR2
FFAR2 Polyclonal Antibody for WB, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, porc et plus (2)
Applications
WB, IHC, IF, IP, ELISA
Conjugaison
Non conjugué
N° de cat : 19952-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules THP-1, cellules HCT 116, cellules HeLa, tissu hépatique de porc | 
| Résultats positifs en IHC | tissu splénique humain,  tissu de côlon humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. | 
Dilution recommandée
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 | 
| Immunohistochimie (IHC) | IHC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 1 publications below | 
| WB | See 28 publications below | 
| IHC | See 2 publications below | 
| IF | See 4 publications below | 
| IP | See 1 publications below | 
Informations sur le produit
19952-1-AP cible FFAR2 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, porc
| Réactivité | Humain, porc | 
| Réactivité citée | rat, Humain, porc, souris | 
| Hôte / Isotype | Lapin / IgG | 
| Clonalité | Polyclonal | 
| Type | Anticorps | 
| Immunogène | Peptide | 
| Nom complet | free fatty acid receptor 2 | 
| Masse moléculaire calculée | 37 kDa | 
| Poids moléculaire observé | 50 kDa, 37 kDa | 
| Numéro d’acquisition GenBank | NM_005306 | 
| Symbole du gène | FFAR2 | 
| Identification du gène (NCBI) | 2867 | 
| Conjugaison | Non conjugué | 
| Forme | Liquide | 
| Méthode de purification | Purification par affinité contre l'antigène | 
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol | 
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. | 
Informations générales
Free fatty acid receptors (FFAR) play significant roles in various physiological processes through interaction with their ligands, fatty acids. Free fatty acid receptor 2 (FFAR2, also known as FFA2 or GPR43) is a receptor for short-chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and intestinal immunity (PMID: 12684041). It has been considered a therapeutic target for metabolic and inflammatory conditions (PMID: 23589301). FFAR2 has a calculated molecular weight of 37 kDa and can be glycosylated. The higher apparent molecular weight of 50 kDa has been reported, probably due to glycosylation (PMID: 31707282; 28131568).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for FFAR2 antibody 19952-1-AP | Download protocol | 
| IHC protocol for FFAR2 antibody 19952-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Food Chem X Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice. | ||
| Chin Med The relationship between gut microbiota and susceptibility to type 2 diabetes mellitus in rats | ||
| Chin Med Shaoyao Decoction reduced T lymphocyte activation by regulating of intestinal flora and 5-hydroxytryptamine metabolism in ulcerative colitis | ||
| Pharmaceuticals (Basel) Xiao Cheng Qi Decoction, an Ancient Chinese Herbal Mixture, Relieves Loperamide-Induced Slow-Transit Constipation in Mice: An Action Mediated by Gut Microbiota | ||
| Phytomedicine The beneficial effects of Polygonatum sibiricum Red. superfine powder on metabolic hypertensive rats via gut-derived LPS/TLR4 pathway inhibition | ||
| Food Funct EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier. | 
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Christin (Verified Customer) (01-26-2025) | Antibody used to study G protein coupled receptors responsible for uptake of short chain fatty acids in human iPSC-derived microglia and macrophages. 
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