Anticorps Monoclonal anti-FFAR3

FFAR3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D10B7

N° de cat : 66811-1-PBS

Synonymes

GPR41, 1D10B7, FFA3R, free fatty acid receptor 3, G protein coupled receptor 41

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Galerie de données de validation

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  WB analysis using 66811-1-Ig (same clone as 66811-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

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  WB analysis of human adipose using 66811-1-Ig (same clone as 66811-1-PBS)

  human adipose tissue were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66811-1-Ig (same clone as 66811-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66811-1-Ig (same clone as 66811-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66811-1-Ig (FFAR3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66811-1-Ig (same clone as 66811-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 66811-1-Ig (FFAR3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66811-1-PBS cible FFAR3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FFAR3 Protéine recombinante Ag28025
• Nom complet: free fatty acid receptor 3
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 40-48 kDa
• Numéro d’acquisition GenBank: BC035657
• Symbole du gène: FFAR3
• Identification du gène (NCBI): 2865
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FFAR3 (Free fatty acid receptors 3) also known as GPR41, with another GPR43 protein, both are Gai-coupled receptor activated by short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate (PMID:26870043). GPR41 protein is translated from the bicistronic mRNA encoding GPR40 and GPR41, where an internal ribosome entry site (IRES) is utilized for the GPR41 coding sequence downstream of GPR40 (PMID:22493486). GPR41 is expressed in adipose tissue, gut, and the peripheral nervous system, and it is involved in SCFA-dependent energy regulation(PMID: 24904531).

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