Anticorps Recombinant de lapin anti-FGD1

• Phare
• Validé par KD/KO

FGD1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242314C3

N° de cat : 84783-2-RR

Synonymes

242314C3, FGDY, FYVE, RhoGEF and PH domain-containing protein 1, Rho/Rac GEF, ZFYVE3

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Galerie de données de validation

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  WB analysis using 84783-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84783-2-RR (FGD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 84783-2-RR

  WB result of FGD1 antibody (84783-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FGD1 transfected U2OS cells.

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  Affinity and Kinetic Characterization of 84783-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84783-2-RR against Human FGD1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: SK-N-SH cells, cellules BxPC-3, cellules MG U-87, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84783-2-RR cible FGD1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FGD1 Protéine recombinante Ag3861
• Nom complet: FYVE, RhoGEF and PH domain containing 1
• Masse moléculaire calculée: 901 aa, 91 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC034530
• Symbole du gène: FGD1
• Identification du gène (NCBI): 2245
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039).

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