Anticorps Polyclonal de lapin anti-FGD5

• Phare
• Validé par KD/KO

FGD5 Polyclonal Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 20910-1-AP

Synonymes

FGD5, ZFYVE23

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Galerie de données de validation

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  IHC staining of human lung using 20910-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20910-1-AP (FGD5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 20910-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20910-1-AP (FGD5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 20910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20910-1-AP (FGD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20910-1-AP (FGD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20910-1-AP

  Immunofluorescent analysis of HepG2 cells, using FGD5 antibody 20910-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20910-1-AP

  Immunofluorescent analysis of Hela cells, using FGD5 antibody 20910-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en IHC: tissu pulmonaire humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

20910-1-AP cible FGD5 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FGD5 Protéine recombinante Ag15037
• Nom complet: FYVE, RhoGEF and PH domain containing 5
• Masse moléculaire calculée: 1462 aa, 160 kDa
• Numéro d’acquisition GenBank: BC035364
• Symbole du gène: FGD5
• Identification du gène (NCBI): 152273
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FGD5 (or ZFYVE23) is an endothelial specific gene, plays a crucial role in vascular pruning. Deficient expression of FGD5 markedly inhibits neovascularization, indicated by in vitro tube-formation, aortic-ring, and coated-bead assays and by in vivo coated-bead plug assays and studies in the murine retina model. FGD5-deficient endothelial cells fail to migrate. Moreover, the cells are susceptible to proapoptotic stimuli associated with VEGF-stimulated activation of Akt. FGD5 promotes apoptosis-induced vaso-obliteration via induction of the hey1-p53 pathway by direct binding and activation of CDC42.

Publications

Species	Application	Title
human,mouse	WB,IF,CoIP	EMBO Rep; VE-PTP inhibition stabilizes endothelial junctions by activating FGD5.; Authors - Laura J Braun ; KD Validated
human	WB	Arterioscler Thromb Vasc Biol; Facio-genital dysplasia-5 regulates matrix adhesion and survival of human endothelial cells.; Authors - Nakhaei-Nejad Maryam M
human	WB,IP	Arterioscler Thromb Vasc Biol; FGD5 Regulates VEGF Receptor-2 Coupling to PI3 Kinase and Receptor Recycling.; Authors - Maikel A Farhan ; KD Validated
human		EMBO Rep; VE-PTP inhibition stabilizes endothelial junctions by activating FGD5.; Authors - Laura J Braun
mouse	IF	Dev Cell; FGD5 in basal cells induces CXCL14 secretion that initiates a feedback loop to promote murine mammary epithelial growth and differentiation; Authors - Tingting Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (11-05-2023)

Antibody performs poorly via western blot with using Near Infrared IRDye secondary antibodies.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

FGD5 Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:20910-1-AP)

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