Anticorps Polyclonal de lapin anti-FGF-2

FGF-2 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11234-1-PBS

Synonymes

FGF2, FGF 2, FGF B, FGF basic, FGFB

---

Galerie de données de validation

• 

  WB analysis using 11234-1-AP (same clone as 11234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11234-1-AP (FGF-2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 11234-1-AP (same clone as 11234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 11234-1-AP (same clone as 11234-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FGF-2 (11234-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 11234-1-AP (same clone as 11234-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human FGF-2 (11234-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

11234-1-PBS cible FGF-2 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: fibroblast growth factor 2 (basic)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: NM_002006
• Symbole du gène: FGF2
• Identification du gène (NCBI): 2247
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

{{ptg:RelatedPrimaryAntibodies}}