Anticorps Polyclonal de lapin anti-FGF12

• Phare
• Validé par KD/KO

FGF12 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 13784-1-AP

Synonymes

FGF 12, FGF12, FGF12B, FHF 1, FHF1, Myocyte activating factor

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Galerie de données de validation

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  WB analysis using 13784-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13784-1-AP (FGF12 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13784-1-AP

  IP result of anti-FGF12 (IP:13784-1-AP, 4ug; Detection:13784-1-AP 1:6000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse brain using 13784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13784-1-AP (FGF12 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 13784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13784-1-AP (FGF12 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

13784-1-AP cible FGF12 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FGF12 Protéine recombinante Ag4797
• Nom complet: fibroblast growth factor 12
• Masse moléculaire calculée: 181 aa, 20 kDa
• Poids moléculaire observé: 20-22 kDa
• Numéro d’acquisition GenBank: BC022524
• Symbole du gène: FGF12
• Identification du gène (NCBI): 2257
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FGF12 is a member of the FGF family. FGF family members play important roles in embryogenesis, angiogenesis, and wound repair. FGF12 lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. FGF12 plays an intracellular role in the inhibition of radiation-induced apoptosis. FGF12 is expressed abundantly in developing and adult nervous systems; therefore, FGF12 was thought to be related to nervous system development and function.

Publications

Species	Application	Title
rat	IF	Neurobiol Dis; Decreased expression of synaptic genes in the vestibular ganglion of rodents following subchronic ototoxic stress; Authors - Erin A Greguske
human	WB	Epilepsia; Early onset epilepsy and sudden unexpected death in epilepsy with cardiac arrhythmia in mice carrying the early infantile epileptic encephalopathy 47 gain-of-function FHF1(FGF12) missense mutation.; Authors - Jana Velíšková
mouse	WB,IF	Sci Rep; Fibroblast growth factor 12 is expressed in spiral and vestibular ganglia and necessary for auditory and equilibrium function.; Authors - Yukiko Hanada ; KO Validated
human	IHC	Heliyon; Identification of cancer-associated fibroblast-related Ectodysplasin-A as a novel indicator for prognosis and immune response in gastric cancer; Authors - Ya Zhang
mouse	WB	Nat Commun; Proximity analysis of native proteomes reveals phenotypic modifiers in a mouse model of autism and related neurodevelopmental conditions; Authors - Yudong Gao

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