Anticorps Recombinant de lapin anti-FGF19

FGF19 Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241740E4

N° de cat : 84536-3-PBS

Synonymes

UNQ334/PRO533, Fibroblast growth factor 19, FGF-19, FGF 19, 241740E4

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Galerie de données de validation

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  WB analysis of HT-29 using 84536-3-RR (same clone as 84536-3-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 84536-3-RR (FGF19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84536-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01398-1

  Sandwich ELISA standard curve of MP01398-1, Human FGF19 Recombinant Matched Antibody Pair - PBS only. 84536-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1330. 84536-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-1000 pg/mL.

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  Sample test of MP01398-1

  Serum of six individual healthy human donors was measured. The FGF19 concentration of detected samples was determined to be 199.4 pg/mL with a range of 136.6-250.1 pg/mL.

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  Sample test of MP01398-1

  HT-29 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human FGF19, and measured 32.7 ng/mL.

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  Cytometric bead array standard curve of MP01398-1

  Cytometric bead array standard curve of MP01398-1, FGF19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84536-3-PBS. Detection antibody: 84536-2-PBS. Standard: HZ-1330. Range: 0.078-20 ng/mL.

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  Affinity and Kinetic Characterization of 84536-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84536-3-RR against Human FGF19 were performed. The affinity constant is 0.323 nM.

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Informations sur le produit

84536-3-PBS cible FGF19 dans les applications de WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FGF19 Protéine recombinante HZ-1330
• Nom complet: fibroblast growth factor 19
• Masse moléculaire calculée: 216 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC017664
• Symbole du gène: FGF19
• Identification du gène (NCBI): 9965
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fibroblast growth factor 19 (FGF19) is a member of the fibroblast growth factor family, which consists of proteins with diverse effects on development, organogenesis, and metabolism. FGF19 is unique within this family as it functions as an endocrine protein, circulating in the blood and acting on distant target tissues. It is produced in the distal ileum and secreted into the circulation after a meal, playing a role in controlling the enterohepatic circulation of bile acids and regulating hepatic protein and glycogen metabolism in an insulin-independent manner. .

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