Anticorps Polyclonal de lapin anti-FGF-21

FGF-21 Polyclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 26272-1-AP

Synonymes

FGF21, FGF 21, Fibroblast growth factor 21, UNQ3115/PRO10196

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Galerie de données de validation

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  IHC staining of human lung cancer using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse liver using 26272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FGF21 antibody (26272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse liver using 26272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FGF21 antibody (26272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

26272-1-AP cible FGF-21 dans les applications de IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FGF-21 Protéine recombinante Ag24064
• Nom complet: fibroblast growth factor 21
• Masse moléculaire calculée: 22 kDa
• Numéro d’acquisition GenBank: BC018404
• Symbole du gène: FGF21
• Identification du gène (NCBI): 26291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibroblast growth factor 21 (FGF21), a member of the FGF superfamily, has emerged as an important metabolic regulator. FGF21 is produced in the liver, adipocytes and skeletal muscle and regulates glucose, energy and lipid metabolism. FGF21 is a stress-responsive hepatokine which is induced in the liver as a response to injury, regeneration, hepatocarcinogenesis and during kidney dysfunction. FGF21 is primarily metabolized in the kidney and renal secretion is the major route of FGF21 elimination. Its plasma levels increase according to the decline in renal function in patients with chronic and acute renal dysfunction.

Publications

Species	Application	Title
mouse		Nat Commun; The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury; Authors - Hakjoo Lee
human		Biomed Pharmacother; Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway.; Authors - Xingran Chen
rat	IF	Cell Death Dis; FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.; Authors - Kailiang Zhou
mouse		Sci Rep; Brown adipose tissue-derived FGF21 mediates the cardioprotection of dexmedetomidine in myocardial ischemia/reperfusion injury; Authors - Yi Ding

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