Anticorps Polyclonal de lapin anti-FGF8

FGF8 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 20711-1-AP

Synonymes

AIGF, Androgen induced growth factor, FGF 8, FGF8, Fibroblast growth factor 8, HBGF 8, KAL6

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Galerie de données de validation

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  WB analysis using 20711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20711-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20711-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20711-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, cellules L02, tissu hépatique de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

20711-1-AP cible FGF8 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: fibroblast growth factor 8 (androgen-induced)
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: NM_006119
• Symbole du gène: FGF8
• Identification du gène (NCBI): 2253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FGF8, also named as AIGF and HBGF-8, belongs to the heparin-binding growth factors family. FGF8 stimulates growth of the cells in an autocrine manner. It mediates hormonal action on the growth of cancer cells. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). The antibody is specific to FGF8.

Publications

Species	Application	Title
human	WB,IHC	Carcinogenesis; The oncoprotein HBXIP enhances angiogenesis and growth of breast cancer through modulating FGF8 and VEGF.; Authors - Fabao Liu
human	IF	Oncoimmunology; Immunoreactivity against fibroblast growth factor 8 in alveolar rhabdomyosarcoma patients and its involvement in tumor aggressiveness.; Authors - Elena Poli
human	IHC	Oncol Lett; Systematic analysis of expression profiles and prognostic significance of the FGF gene family in pancreatic adenocarcinoma; Authors - Yu-Xin Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paolo (Verified Customer) (12-12-2019)

It works fine in wb, in IF differences in FGF8 intracellular localization were observed according to the fixative solution used

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1/500