Anticorps Polyclonal de lapin anti-FGF9

FGF9 Polyclonal Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 26554-1-AP

Synonymes

FGF-9, FGF 9, Heparin-binding growth factor 9, HBGF-9, Glia-activating factor

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Galerie de données de validation

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  IHC staining of human stomach using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26554-1-AP (FGF9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26554-1-AP (FGF9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 26554-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FGF9 antibody (26554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26554-1-AP cible FGF9 dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FGF9 Protéine recombinante Ag24244
• Nom complet: fibroblast growth factor 9 (glia-activating factor)
• Masse moléculaire calculée: 208 aa, 23 kDa
• Numéro d’acquisition GenBank: BC069692
• Symbole du gène: FGF9
• Identification du gène (NCBI): 2254
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibroblast growth factor 9 (FGF9) is a critically important and elegantly regulated growth factor that was first identified during a screen for factors acting on cells of the central nervous system (CNS). Over the subsequent two decades of research, FGF9 has been revealed to play a significant role in various biological processes. A key feature of its control is reciprocal compartmentalization, particularly during development, with the epithelium serving as a dominant source and mesenchyme as a prime target. This mesenchyme selectivity is achieved through the high affinity of FGF9 to the IIIc isoforms of FGFR1, 2, and 3. FGF9 is widely expressed in the embryo, including in the developing heart and lungs, and more selectively in adult tissues, such as the CNS and kidneys.

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