Anticorps Monoclonal anti-FGFR3

• Phare
• Validé par KD/KO

FGFR3 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

1F3G1

Avis (1)

N° de cat : 66954-1-Ig

Synonymes

ACH, CD333, CEK2, FGFR 3, FGFR3, HSFGFR3EX, JTK4

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Galerie de données de validation

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  WB analysis using 66954-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66954-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L-929 using 66954-1-Ig

  L-929 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66954-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66954-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66954-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 66954-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66954-1-Ig (FGFR3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66954-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGFR3 antibody (66954-1-Ig, Clone: 1F3G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66954-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGFR3 antibody (66954-1-Ig, Clone: 1F3G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules L02, cellules L-929, cellules NCI-H1299
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

66954-1-Ig cible FGFR3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FGFR3 Protéine recombinante Ag26290
• Nom complet: fibroblast growth factor receptor 3
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 125-135 kDa
• Numéro d’acquisition GenBank: NM_000142
• Symbole du gène: FGFR3
• Identification du gène (NCBI): 2261
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3 and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, FGFR3 gene has also been associated with tumor progression.

Publications

Species	Application	Title
mouse	WB	Ann Rheum Dis; FGFR3 deficiency enhances CXCL12-dependent chemotaxis of macrophages via upregulating CXCR7 and aggravates joint destruction in mice.; Authors - Liang Kuang ; KD Validated
human	WB	Cells; Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.; Authors - Damian Kołat
mouse	WB	Bioengineered; The effects of Omarigliptin on promoting osteoblastic differentiation.; Authors - Fake Liao
human	WB	J Exp Clin Cancer Res; HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway.; Authors - Kai Guo
human	WB	J Exp Clin Cancer Res; Dysregulation and prometastatic function of glycosyltransferase C1GALT1 modulated by cHP1BP3/ miR-1-3p axis in bladder cancer.; Authors - Zengqi Tan
human	IF	Tissue Eng Part A; The UC-MSCbFGF/scaffold system accelerates the healing of the uterine full-thickness injury; Authors - Limin Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Clarisse (Verified Customer) (12-12-2022)

This antibody works very well on tissue fixed overnight at 4C with 2% PFA. No antigen retrieval required.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:750
• Cell Tissue Type: Human cerebral organoids