Anticorps Monoclonal anti-FGFR3

• Phare
• Validé par KD/KO

FGFR3 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F3G1

N° de cat : 66954-1-PBS

Synonymes

JTK4, HSFGFR3EX, FGFR 3, CEK2, CD333

---

Galerie de données de validation

• 

  WB analysis using 66954-1-Ig (same clone as 66954-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66954-1-Ig (same clone as 66954-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L-929 using 66954-1-Ig (same clone as 66954-1-PBS)

  L-929 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66954-1-Ig (same clone as 66954-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66954-1-Ig (same clone as 66954-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGFR3 antibody (66954-1-Ig, Clone: 1F3G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGFR3 antibody (66954-1-Ig, Clone: 1F3G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50578-1

  Cytometric bead array standard curve of MP50578-1, FGFR3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66954-1-PBS. Detection antibody: 66954-2-PBS. Standard:Ag26290. Range: 1.563-200 ng/mL.

---

Informations sur le produit

66954-1-PBS cible FGFR3 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FGFR3 Protéine recombinante Ag26290
• Nom complet: fibroblast growth factor receptor 3
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 125-135 kDa
• Numéro d’acquisition GenBank: NM_000142
• Symbole du gène: FGFR3
• Identification du gène (NCBI): 2261
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3, and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, the FGFR3 gene has also been associated with tumor progression.

{{ptg:RelatedPrimaryAntibodies}}