Anticorps Polyclonal de lapin anti-FGFR4

• Phare
• Validé par KD/KO

FGFR4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(19)

Avis (1)

N° de cat : 11098-1-AP

Synonymes

CD334, EC:2.7.10.1, FGFR 4, FGFR-4, JTK2

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Galerie de données de validation

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  WB analysis using 11098-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11098-1-AP (FGFR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11098-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11098-1-AP (FGFR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11098-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11098-1-AP (FGFR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11098-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11098-1-AP (FGFR4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 7 publications below

Informations sur le produit

11098-1-AP cible FGFR4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FGFR4 Protéine recombinante Ag1573
• Nom complet: fibroblast growth factor receptor 4
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 95, 100-110 kDa
• Numéro d’acquisition GenBank: BC011847
• Symbole du gène: FGFR4
• Identification du gène (NCBI): 2264
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibroblast growth factor receptor 4 (FGFR4) is a member of a highly conserved tyrosine kinase family, along with FGFR1-3. This family consists of an intracellular tyrosine kinase domain, a single transmembrane domain, and extracellular ligand-binding domains (PMID: 32492514). FGFR4 is the predominant FGFR isoform present in human hepatocytes. FGFR4 has been proposed to play a role in the observed induction of hepatocyte proliferation and carcinogenesis by FGF19; however, contradicting evidence proposing a protective role for FGFR4 in suppressing hepatoma progression has also been proposed(PMID: 20018895). While the role of FGFR4 in cancer remains to be fully elucidated, several findings suggest that this receptor may be an important player in Hepatocellular carcinoma (HCC) development and/or progression(PMID: 10336501).

Publications

Species	Application	Title
mouse,human	WB,IHC	J Med Chem; FGF19-Based Mini Probe Targeting FGFR4 for Diagnosis and Surgical Navigation of Hepatocellular Carcinoma; Authors - Jiaxian Cai ; KD Validated
mouse	WB	Biochem Pharmacol; Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.; Authors - Yanan Chen
mouse	WB	Biomed Pharmacother; Bupleuri radix extract ameliorates impaired lipid metabolism in high-fat diet-induced obese mice via gut microbia-mediated regulation of FGF21 signaling pathway.; Authors - Liucen Wu
human		J Cell Biochem; Glycosylation of FGFR4 in cholangiocarcinoma regulates receptor processing and cancer signaling.; Authors - Andrew J Phillips
human	WB,IHC	Int J Oncol; Role of fibroblast growth factor 4 in the growth and metastasis of colorectal cancer.; Authors - Yanwei Ye
human	IHC	Cancer Res Treat; FGFR4 Gly388Arg Polymorphism Affects the Progression of Gastric Cancer by Activating STAT3 Pathway to Induce Epithelial to Mesenchymal Transition.; Authors - Yanwei Ye

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jillian (Verified Customer) (05-27-2025)

Overall we weren't as happy with the antibody as we wanted to be. There appeared to be a lot of nonspecific binding in the NHP samples that we tested it on

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Liver, Frontal, Temporal

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