Anticorps Polyclonal de lapin anti-FGL1

• Phare
• Validé par KD/KO

FGL1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 16000-1-AP

Synonymes

FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, HPS, LFIRE 1, LFIRE1

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Galerie de données de validation

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  WB analysis using 16000-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16000-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16000-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 16000-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16000-1-AP

  IP Result of anti-FGL1 (IP:16000-1-AP, 4ug; Detection:16000-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human liver cancer using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules HepG2, tissu de thymus de souris, tissu hépatique humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cirrhose hépatique humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 11 publications below
• IF: See 2 publications below

Informations sur le produit

16000-1-AP cible FGL1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FGL1 Protéine recombinante Ag8581
• Nom complet: fibrinogen-like 1
• Masse moléculaire calculée: 312 aa, 36 kDa
• Poids moléculaire observé: 34 kDa, 68 kDa
• Numéro d’acquisition GenBank: BC007047
• Symbole du gène: FGL1
• Identification du gène (NCBI): 2267
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FGL1 is a 68 kDa protein that comprises two disulfide linked 34 kDa homodimers. FGL1 is a predominantly liver expressed protein that has been implicated as both a hepatoprotectant and a hepatocyte mitogen. FGL1 expression is decreased in hepatocellular carcinoma (HCC), and it may play a role in the development of hepatocellular carcinomas.

Publications

Species	Application	Title
human	WB,IHC	Transl Res; Role of placental fibrinogen-like protein 1 in gestational diabetes.; Authors - Lin Kang
mouse	IHC	Front Immunol; Fibrinogen-like protein 1 promotes liver-resident memory T-cell exhaustion in hepatocellular carcinoma; Authors - Changjie Yang
mouse	WB	Diabetologia; PMID: 27221093; Authors - Hung-Tsung Wu ; KD Validated
mouse	WB,IHC	J Nanobiotechnology; Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles.; Authors - Chunai Gong
human	WB	Int J Mol Sci; Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells.; Authors - Yeonghoon Son
human,mouse	WB	J Clin Endocrinol Metab; The Hepatic Protection Effects of Hepassocin in Hyperglycemic Crisis.; Authors - Horng-Yih Ou