Anticorps Recombinant de lapin anti-FGR

FGR Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240275E5

N° de cat : 83352-1-RR

Synonymes

p58c fgr, p55c fgr, p55 Fgr, c src2, c fgr

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Galerie de données de validation

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  WB analysis using 83352-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83352-1-RR (FGR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Raji using 83352-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using FGR antibody (83352-1-RR, Clone: 240275E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of Raji using 83352-1-RR

  1x10^6 Raji cells were intracellularly stained with 0.25 ug FGR Recombinant antibody (83352-1-RR, Clone:240275E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83352-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83352-1-RR against Human FGR were performed. The affinity constant is 0.139 nM.

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji
• Résultats positifs en IF/ICC: cellules Raji,
• Résultats positifs en FC (Intra): cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83352-1-RR cible FGR dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FGR Protéine recombinante Ag34944
• Nom complet: Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_005248
• Symbole du gène: FGR
• Identification du gène (NCBI): 2268
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FGR (Tyrosine-protein kinase Fgr) is a member of the Src family of protein tyrosine kinases (PTKs). It's contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively.

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