Anticorps Polyclonal de lapin anti-FH

FH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 11375-1-AP

Synonymes

FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1

---

Galerie de données de validation

• 

  WB analysis using 11375-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11375-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11375-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11375-1-AP

  IP result of anti-FH(IP:11375-1-AP, 4ug; Detection:11375-1-AP 1:8000) with mouse liver tissue lysate 3040 ug.

• 

  IHC staining of human placenta using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11375-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FH antibody (11375-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C6, cellules HeLa, cellules HepG2, cellules Jurkat, cellules L02, cellules NIH/3T3, tissu hépatique de rat, tissu hépatique de souris, tissu placentaire humain
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu placentaire humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 2 publications below

Informations sur le produit

11375-1-AP cible FH dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Caenorhabditis elegans
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FH Protéine recombinante Ag1934
• Nom complet: fumarate hydratase
• Masse moléculaire calculée: 510 aa, 55 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC003108
• Symbole du gène: FH
• Identification du gène (NCBI): 2271
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

Publications

Species	Application	Title
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
human	WB	Theranostics; EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter.; Authors - Feng Shi
human	WB	Oncotarget; CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.; Authors - Zhichao Gu
human	WB	Invest Ophthalmol Vis Sci; Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss.; Authors - Coupland Sarah E SE
Caenorhabditis elegans	WB	Int J Radiat Biol; Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.; Authors - Yongyan Sun